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anti psgl 1 fitc  (R&D Systems)


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    R&D Systems anti psgl 1 fitc
    Anti Psgl 1 Fitc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+psgl+1+fitc/Human+PSGL-1%2FCD162+Alexa+Fluor%C2%AE+488-conjugated+Antibody/pm39507948-85-29-31
    Average 93 stars, based on 3 article reviews
    anti psgl 1 fitc - by Bioz Stars, 2026-09
    93/100 stars

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    Staining:

    Article Title: Pathomechanisms of HIV-Associated Cerebral Small Vessel Disease: A Comprehensive Clinical and Neuroimaging Protocol and Analysis Pipeline
    Article Snippet: .. The cells are then washed and stained with titrated amounts of antibodies against anti-CD14 PE (BD Biosciences # 555398; 10 μl), anti-CD16 PE Cy7 (BD Biosciences 557744; 3 μl), anti-CD61AF647 (Biolegend # 336408; 3 μl), anti-PSGL-1 FITC (R&D Systems # FAB9961G; 1.5 μl), anti-CD40 FITC BD Biosciences. .. # 555598; 10 μl), anti-CCR2 FITC (R&D Systems # FAB151G; 1.5 μl), anti-CD62P FITC (BD Biosciences # 555523; 5 μl), and anti-TNFR2 FITC (Miltenyi Biotech #130-107-743; 1 μl) for 30 min at room temperature in the dark.

    Article Title: Non-classical monocyte levels correlate negatively with HIV-associated cerebral small vessel disease and cognitive performance.
    Article Snippet: .. The cells were then washed and stained with titrated amounts of antibodies against anti-CD14 PE (BD Biosciences # 555398; 10 ml), anti-CD16 PE Cy7 (BD Biosciences 557744; 3 ml), anti-PSGL-1 FITC (R&D Systems # FAB9961G; 1.5 ml), anti-CD40 FITC BD Biosciences. .. # 555598; 10 ml), anti-CCR2 FITC (R&D Systems # FAB151G; 1.5 ml), anti-TF FITC (Miltenyi Biotech Inc # REA949, 10ml) and anti-TNFR2 FITC (Miltenyi Biotech #130-107- 743; 1ml) for 30 minutes at room temperature in dark.

    Article Title: Role of non-classical monocytes in HIV-associated vascular cognitive impairment
    Article Snippet: .. The cells were then washed and stained with titrated amounts of antibodies against anti-CD14 PE (BD Biosciences # 555398; 10 ul), anti-CD16 PE Cy7 (BD Biosciences 557744; 3 ul), anti-CD61AF647 (Biolegend # 336408; 3 ul), anti-PSGL-1 FITC (R&D Systems # FAB9961G; 1.5 ul), anti-CD40 FITC BD Biosciences. .. # 555598; 10 ul), anti-CCR2 FITC (R&D Systems # FAB151G; 1.5 ul), anti-CD62P FITC (BD Biosciences # 555523; 5 ul) and anti-TNFR2 FITC (Miltenyi Biotech #130-107-743; 1 ul) for 30 minutes at room temperature in dark.

    Article Title: Non-classical monocyte levels correlate negatively with HIV-associated cerebral small vessel disease and cognitive performance
    Article Snippet: .. The cells were then washed and stained with titrated amounts of antibodies against anti-CD14 PE (BD Biosciences # 555398; 10 μl), anti-CD16 PE Cy7 (BD Biosciences 557744; 3 μl), anti-PSGL-1 FITC (R&D Systems # FAB9961G; 1.5 μl), anti-CD40 FITC BD Biosciences. .. # 555598; 10 μl), anti-CCR2 FITC (R&D Systems # FAB151G; 1.5 μl), anti-TF FITC (Miltenyi Biotech Inc # REA949, 10μl) and anti-TNFR2 FITC (Miltenyi Biotech #130-107-743; 1μl) for 30 minutes at room temperature in dark.



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    Image Search Results


    Suspensions of isolated neutrophils were perfused through straight (St) and a constricted (310 msec) channel microfluidic devices, the effluents collected and labeled with an anti-PSGL-1 antibody (PL1) followed by a secondary antibody, and the expression level of PSGL-1 on the surface of neutrophils was analyzed by flow cytometry. Unsheared (Un) samples or samples incubated with 10nM PMA served as negative and positive controls. The data is shown as the mean fluorescent intensity ± standard error of the mean and is representative of five independent experiments with different donors. Statistical significance of the differences between the samples and the unsheared sample was measured using ANOVA with a Sidak post hoc test (*p<0.05).

    Journal: Cellular and Molecular Bioengineering

    Article Title: Effects of Transient Exposure to High Shear on Neutrophil Rolling Behavior

    doi: 10.1007/s12195-018-0533-z

    Figure Lengend Snippet: Suspensions of isolated neutrophils were perfused through straight (St) and a constricted (310 msec) channel microfluidic devices, the effluents collected and labeled with an anti-PSGL-1 antibody (PL1) followed by a secondary antibody, and the expression level of PSGL-1 on the surface of neutrophils was analyzed by flow cytometry. Unsheared (Un) samples or samples incubated with 10nM PMA served as negative and positive controls. The data is shown as the mean fluorescent intensity ± standard error of the mean and is representative of five independent experiments with different donors. Statistical significance of the differences between the samples and the unsheared sample was measured using ANOVA with a Sidak post hoc test (*p<0.05).

    Article Snippet: FITC tagged mouse-anti-PSGL-1 mAb PL1 was purchased from MBL (Woburn, MA), while unlabeled PL1 was purchased from Ancell (Bayport, MN).

    Techniques: Isolation, Labeling, Expressing, Flow Cytometry, Incubation