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anti psgl 1 fitc  (R&D Systems)


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    R&D Systems anti psgl 1 fitc
    Anti Psgl 1 Fitc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+psgl+1+fitc/pm39507948-85-29-31?v=R%26D+Systems
    Average 93 stars, based on 3 article reviews
    anti psgl 1 fitc - by Bioz Stars, 2026-08
    93/100 stars

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    Suspensions of isolated neutrophils were perfused through straight (St) and a constricted (310 msec) channel microfluidic devices, the effluents collected and labeled with an anti-PSGL-1 antibody (PL1) followed by a secondary antibody, and the expression level of PSGL-1 on the surface of neutrophils was analyzed by flow cytometry. Unsheared (Un) samples or samples incubated with 10nM PMA served as negative and positive controls. The data is shown as the mean fluorescent intensity ± standard error of the mean and is representative of five independent experiments with different donors. Statistical significance of the differences between the samples and the unsheared sample was measured using ANOVA with a Sidak post hoc test (*p<0.05).

    Journal: Cellular and Molecular Bioengineering

    Article Title: Effects of Transient Exposure to High Shear on Neutrophil Rolling Behavior

    doi: 10.1007/s12195-018-0533-z

    Figure Lengend Snippet: Suspensions of isolated neutrophils were perfused through straight (St) and a constricted (310 msec) channel microfluidic devices, the effluents collected and labeled with an anti-PSGL-1 antibody (PL1) followed by a secondary antibody, and the expression level of PSGL-1 on the surface of neutrophils was analyzed by flow cytometry. Unsheared (Un) samples or samples incubated with 10nM PMA served as negative and positive controls. The data is shown as the mean fluorescent intensity ± standard error of the mean and is representative of five independent experiments with different donors. Statistical significance of the differences between the samples and the unsheared sample was measured using ANOVA with a Sidak post hoc test (*p<0.05).

    Article Snippet: FITC tagged mouse-anti-PSGL-1 mAb PL1 was purchased from MBL (Woburn, MA), while unlabeled PL1 was purchased from Ancell (Bayport, MN).

    Techniques: Isolation, Labeling, Expressing, Flow Cytometry, Incubation